B016282 - BIOTRANSFORMATIONS IN ORGANIC CHEMISTRY

italian

Biotransformations in organic chemistry, advantages and limitations. Selectivity of the enzymes and models. Use of various classes of enzymes in the laboratory and in industrial organic syntheses with focus on the manipulation of specific functional groups. Oxido-reductases. Glycosyltransferase. Cascade enzyme reactions. Biocatalysis in total synthesis. Examples of non-conventional biotransformations.

Kurt Faber, Biotransformation in Organic Chemistry, A textbook. Springer ed. 2011, Sixth ed.

At the end of the course, the students should:be familiar with the concept of biotranformation/biocatalysisdemonstrate understanding of the advantages and challenges of using biotransformations in synthesis and of the criteria for choosing between biocatalysts and inorganic/organic catalysts.be able to classify enzymes based on reaction types and to choose the appropriate enzyme for a given reaction be aware of recent developments in the field and of its interdisciplinarity

none

frontal lessons

Exercises and other activities with direct participation of the students will be done during the course about the use of enzymes in organic synthesis

Oral exams.

Biotransformations in organic chemistry, advantages and limitations. Selectivity of the enzymes and models. Use of various classes of enzymes in the laboratory and in industrial organic syntheses with focus on the manipulation of specific functional groups. Hydrolases for the hydrolysis of C-N and C-O bonds in amides, hydantoins , esters, lactones, lactames, epoxides and phosphate esters. Use of oxido-reductases in aldehydes and ketones reduction, alcohols oxidation, hydroxylation and dihydroxylation reactions. Lyases and the formation of C-C and CN bonds. Glycosyltransferase for glycosylation of peptides/proteins and small molecules. Cascade enzyme reactions. Biocatalysis in total synthesis. Examples of non-conventional biotransformations: immobilized enzymes, modified enzymes, artificial cells and catalytic antibodies

Objectives: 3, 4, 10, 12