Menù principale
B020179 - ANALYTICAL TECHNIQUES AND DIAGNOSTIC METHODS
Main information
Course Content
Suggested readings
Learning Objectives
Prerequisites
Teaching Methods
Type of Assessment
Course program
Academic Year 2017-18
Course year
Third year - First Semester
Belonging Department
Experimantal and Clinical Medicine
Modulo di sola Frequenza of
Scientific Area
MED/46 - BIOTECHNOLOGY AND METHODS IN LABORATORY MEDICINE
Credits
3
Teaching Hours
36
Teaching Term
02/10/2017 ⇒ 30/04/2019
Attendance required
No
Type of Evaluation
Giudizio Finale
Course Content
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Course program
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Lectureship
Course Content
Immunohematology Laboratory: Red cell antigen typing for blood trasfusion
Immunohematology Laboratory: Laboratory Investigation for Autoimmune Emolytic Anemia (AEA)
Immunohematology Laboratory: Laboratory Investigation for Neonatal Emolytic Anemia (MEN)
Immunohematology Laboratory: Laboratory Investigation for Autoimmune Emolytic Anemia (AEA)
Immunohematology Laboratory: Laboratory Investigation for Neonatal Emolytic Anemia (MEN)
Suggested readings (Search our library's catalogue)
Technical Manual 15th Edition
Learning Objectives
To identify the criteria for evaluating and interpreting the main tests used in the immunohematology laboratory
Knowledge and understanding:
Know the main tests used in the Immunohematology Lab
Know the immunohematological investigations required for transfusion
Know the erythrocyte typing
To know the laboratory diagnostics of autoimmune hemolytic anemia (AEA) and Neonatal Emolytic Anemia (MEN)
Ability to apply knowledge and understanding:
Evaluate the appropriateness of immunohematology labs for erythrocyte typing and erythrocyte transfusion
To evaluate the appropriateness of immunohematology labs for laboratory diagnostics of autoimmune hemolytic anemia (AEA) and Neonatal Emolytic Anemia (MEN)
Knowledge and understanding:
Know the main tests used in the Immunohematology Lab
Know the immunohematological investigations required for transfusion
Know the erythrocyte typing
To know the laboratory diagnostics of autoimmune hemolytic anemia (AEA) and Neonatal Emolytic Anemia (MEN)
Ability to apply knowledge and understanding:
Evaluate the appropriateness of immunohematology labs for erythrocyte typing and erythrocyte transfusion
To evaluate the appropriateness of immunohematology labs for laboratory diagnostics of autoimmune hemolytic anemia (AEA) and Neonatal Emolytic Anemia (MEN)
Prerequisites
None
Teaching Methods
Frontal lessons
Type of Assessment
Written final exam
Course program
1) red cell antigen typing
- Evolution of Transfusion Medicine: Historical data
- Characteristics of the main group-blood systems and their determination in the laboratory:
the ABO system:
description and formation of antigens A and B,
genotype and phenotype ABO,
phenotype Bombay,
compatibility chart for blood transfusion,
Compatibility chart for transfusion of platelets
Laboratory for ABO Determination
The Rh system:
Historical data
Rh and MEN factor
Antigen D
Antigens C, E, C, and
Rh system genetics
genotype and phenotype Rh
D partial
Rh determination in the laboratory
Other blood groups: Extended typing
MNS and U system:
glycophorines A and B,
genetics
Antibodies to the MNS system
Kell System:
The KEL1 antigen and the different Kell antigens (KEL2, KEL3, KEL4, KEL6, KEL7, Kx and Mc Leod's antigen)
Kell System Antibodies
Duffy system
Kidd system
Lewis System
2) Direct Coombs Test (TAD) (or Coombs Direct Test = TCD)
Description and procedure
Characteristics of antibodies responsible for positive direct Coombs test and complement activation
Clinical Meaning of Positivity of Direct Coombs test in Autoimmune Hemolytic Anemia (AEA), in MEN, in drug-induced hemolytic anemia and transfusion hemolytic reactions
3) Indirect antiglobulin (TAI) test (or indirect coombs test)
Description and procedure
Clinical significance of positivity (identification of irregular serum anti-erythrocyte antibodies, determination of erythrocytic antigens, consequences for transfusion and pregnancy)
4) Diagnosis of autoimmune haemolytic anemia (AEA)
Description of the various AEAs
Hot type AEA with warm autoantibodies
Cold AEA and cold autoantibodies
Paroxysmal hemoglobinuria from cold. Donath-Landsteiner syndrome
Mixed type AEA
AEA of two-phase type
6) Neonatal Emolytic Anemia (MEN)
Pathophysiology
Therapy (TIU.)
- Evolution of Transfusion Medicine: Historical data
- Characteristics of the main group-blood systems and their determination in the laboratory:
the ABO system:
description and formation of antigens A and B,
genotype and phenotype ABO,
phenotype Bombay,
compatibility chart for blood transfusion,
Compatibility chart for transfusion of platelets
Laboratory for ABO Determination
The Rh system:
Historical data
Rh and MEN factor
Antigen D
Antigens C, E, C, and
Rh system genetics
genotype and phenotype Rh
D partial
Rh determination in the laboratory
Other blood groups: Extended typing
MNS and U system:
glycophorines A and B,
genetics
Antibodies to the MNS system
Kell System:
The KEL1 antigen and the different Kell antigens (KEL2, KEL3, KEL4, KEL6, KEL7, Kx and Mc Leod's antigen)
Kell System Antibodies
Duffy system
Kidd system
Lewis System
2) Direct Coombs Test (TAD) (or Coombs Direct Test = TCD)
Description and procedure
Characteristics of antibodies responsible for positive direct Coombs test and complement activation
Clinical Meaning of Positivity of Direct Coombs test in Autoimmune Hemolytic Anemia (AEA), in MEN, in drug-induced hemolytic anemia and transfusion hemolytic reactions
3) Indirect antiglobulin (TAI) test (or indirect coombs test)
Description and procedure
Clinical significance of positivity (identification of irregular serum anti-erythrocyte antibodies, determination of erythrocytic antigens, consequences for transfusion and pregnancy)
4) Diagnosis of autoimmune haemolytic anemia (AEA)
Description of the various AEAs
Hot type AEA with warm autoantibodies
Cold AEA and cold autoantibodies
Paroxysmal hemoglobinuria from cold. Donath-Landsteiner syndrome
Mixed type AEA
AEA of two-phase type
6) Neonatal Emolytic Anemia (MEN)
Pathophysiology
Therapy (TIU.)