B021419 - PROTEIN PURIFICATION AND CHARACTERIZATION

Italian

Chromatographic and electrophoretic techniques n protein purification and methods for measuring protein concentration. Experimental aspects of the most important techniques in protein characterization: secondary and tertiary structure through circular dichroism and fluorescence. Determination of the molecular mass and protein sequencing through mass spectrometry.

‘Chimica’ I. Bertini, C. Luchinat, F. Mani, Casa Editrice Ambrosiana; ‘Biochimica’ A. L. Lehninger, Zanichelli.

This course aims at providing the basis for the characterization of proteins starting from their purification based on their biochemical properties through chromatographic and electrophoretic techniques. Furthermore, it gives insights into two methods of characterization such as mass spectrometry and circular dichorism.

Knowledge of the fundamentals of general chemistry and of biochemistry.

Lectures and laboratory practicals.

Oral exam

Chromatographic and electrophoretic techniques in protein purification and methods for measuring protein concentration. Experimental aspects of the most important techniques in protein characterization: secondary and tertiary structure through circular dichroism and fluorescence. Determination of the molecular mass and protein sequencing through mass spectrometry.