Menù principale
B016940 - GENETIC ENGINEERING
Main information
Teaching Language
Course Content
Suggested readings
Learning Objectives
Prerequisites
Teaching Methods
Further information
Type of Assessment
Course program
Sustainable Development Goals 2030
Academic Year 2023-24
Course year
Third year - First Semester
Belonging Department
Experimantal and Clinical Medicine
Course Type
Single education field course
Scientific Area
BIO/18 - GENETICS
Credits
6
Teaching Hours
48
Teaching Term
18/09/2023 ⇒ 22/12/2023
Attendance required
No
Type of Evaluation
Final Grade
Course Content
show
Course program
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Lectureship
Teaching Language
Italian (slides in english)
Course Content
Restriction and modification enzymes. PCR. DNA sequencing. Hybridization of DNA. Vectors and plasmids. Reporter genes. Libraries. Heterologous protein production. Methods for genes expression analysis, methylation analysis and study of protein DNA interaction. Methods for the study of genetic polymorphism. Techniques for GMO. Mutagenesis. CRISPR. Synthetic biology
Suggested readings (Search our library's catalogue)
Papers and slides suggested during classes.
Textbooks of genetic engineering:
Primrose, Twiman & Bold, Principles of Gene Manipulation
Brown, Gene Cloning and DNA Analysis: An Introduction
Learning Objectives
Knowledge acquired: Basic and advanced knowledge genetic engineering. Knowledge on methodologies for genetic manipulation of organisms
Competence acquired
The student has acquired competence on the methods for DNA analysis and manipulation
Skills acquired (at the end of the course):
The student is able to autonomously set up an experimental and protocol for DNA analysis.
Prerequisites
Recommended classes on Genetics and Molecular Biology
Teaching Methods
Total hours of the course (including the time spent in attending lectures, seminars, private study, examinations, etc...): 150
Hours reserved to private study and other individual formative activities: 102
Contact hours for: Lectures (hours): 48
Contact hours for: Laboratory (hours):
Contact hours for: Laboratory-field/practice (hours): 0
Seminars (hours): 0
Stages: 0
Intermediate examinations: 0
Further information
Office hours:
Upon request from Monday to Friday
Teaching tools: Additional teaching material will be provided during lectures and through the Moodle platform
Type of Assessment
Exam modality: written
Duration: 1h
Type of questions: open and closed questions
Aim: verify knowledge and competences (problem solving)
Course program
Restriction and modification enzymes. PCR. DNA sequencing. Hybridization of DNA. Vectors and plasmids. Reporter genes. Libraries. Heterologous protein production. Methods for genes expression analysis, methylation analysis and study of protein DNA interaction. Methods for the study of genetic polymorphism. Techniques for GMO. Mutagenesis. CRISPR. Synthetic biology