B016947 - MOLECULAR BIOLOGY AND BIOINFORMATIC APPLICATIONS

italian

Regulation of gene expression in eukaryotes and techniques for gene expression evaluation. Molecular aspects of the principal biological processes: cell growth, differentiation, cell death and motility and specific techniques for their evaluation.
Biotechnology databanks. Sequence alignment. Information in multiple alignments. Prediction of transcription factors binding sites and and microRNA targets. Gene finding.

Alberts L'essenziale di Biologia molecoalre della cellula Darnell, Lodish: Biologia molecolare della cellula Ed. Zanichelli
T.A. Brown "Genomi" Ed. Edises
Lewin Benjamin; Il gene VIII; Ed. Zanichelli
J. Watson,Biologia Molecolare del Gene . Ed. Zanichelli
Introduzione alla Genomica”
Gibson e Muse; Zanichelli.
Griffith-Zanichelli- Genetica-Principi di analisi Formale
Hartwell- McGraw-Hill- Dall’analisi formale alla genomica
"Fondamenti di Bioinformatica" Citterich et al. Zanichelli

Terminology. Basic aspects and molecular factors involved in the control of the fundamental biological processes: cell growth, differentiation, death, motility and adhesion.
Molecular factors of the signalling pathways leading to cell survive and death.
Bioinformatics

Biochemistry and Biology

CFU: 6
total 52 h
Bioinformatics lab: 12h

The instruments and facilities at the Department of Biomedical Sciences (Biochemistry section) as well as a Laboratory of Cell Biology specific for practical lessons on bioinformatics are used

Students will be evaluated through an oral examination during which the whole course program will be assessed as well as their ability in solving biotecnological topics.

Regulation of gene expression in eukaryotes. Structure and regulation of transcription factors: Chromatin remodelling (Swi/sfn complex, HMG proteins). Transient, semipermanent and permanent gene expression. Cell specification and differentiation. Gene silencing and microRNA. Molecular aspects of the principal biological processes: cell growth, differentiation, cell death and motility. Adesion molecules. Extracellular matrix remodelling. Cytoskeleton: structure and function. Cell communication: gap junctions Methodologies for the evaluation of cell growth, cell death and cell migration.
Batabanks for genomics, transcriptomics and gene regulation. Theory and practice of sequence alignment. Information content in multiple alignments: motifs, signatures, fingerprints, profiles, Markov models, logos, . Methods for transcription factors binding sites identification. Methods for microRNA targets discovery. CpG islands, promoters and gene finding.